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quantitative technique

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1.     First, please answer the questions on the word sheet, attached with the lab report  2.      Second, you need to write lab report about serial dilution experiment, following the direction below:   Please, go along with the format (rubric). Means, the report should have subsequent parts: purpose(objective), material, procedure, results, discussion, and conclusion. Please, see the sample of lab report, that already posted. Please, use following information to build your results, discussion, and conclusion parts.   Let’s assume after doing the experiments you got these results: Plate C 450 colonies which its dilution factor is  10^-4  , Plate D 160 colonies which its dilution factor is  10^-5  , plate E 20 colonies which its dilution factor is  10^-6 , with fact the volume plated 0.1ml.   v Please, use the formula CFU/ml  from the power point slide 18 to count the original Cell Density (OCD) and final dilution factor for each plate.   v   For results part, you can use pictures on the power point     Please, read the power point about Serial dilution, below are the most important videos can help to boost your understanding about serial dilution:   How calculating dilution factor and final dilution factor   https://www.youtube.com/watch?v=egYDN7l4Ka8   How Calculating Colony Forming Unit (CFU Units/ml)   https://www.youtube.com/watch?v=F-L74qIoChA&t=39s   How to create serial dilutions   https://www.youtube.com/watch?v=FdzKgotzjC4   How to prepare spread plates   https://www.youtube.com/watch?v=FwW_2ii1Zoo

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Phyllis Mugure

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